论文部分内容阅读
采用时间分辨荧光光谱测试系统,研究了超高产杂交水稻(Oryza sativa L.)两优培九(P9)和对照汕优63(SH 63)类囊体膜的荧光光谱特性和时间特性。以脉宽为120 ps,重复率为4 MHz,波长为514 nm的Ar+激光分别激发P9和SH 63水稻类囊体膜荧光。通过对其超快荧光的时间特性和光谱特性比较研究发现:无论是在灌浆期还是在扬花期,P9水稻类囊体膜中光系统Ⅰ激发能传递的速度比光系统Ⅱ 的快;P9和SH 63两种水稻类囊体膜在灌浆期的激发能传递速度都比扬花期的快;两种水稻类囊体膜的光谱特性还给出,在从扬花期到灌浆期这一生长发育过程中,SH 63水稻类囊体膜的色素成分和结合状态发生了变化,而P9却没有出现这种变化。
The time-resolved fluorescence spectroscopy system was used to study the fluorescence spectra and time characteristics of the thylakoid membranes of two rice cultivars (Oryza sativa L.) Liangyoupeijiu (P9) and Shanyou63 (SH63). The fluorescence of thylakoid membrane of P9 and SH63 was stimulated by Ar + laser with pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm, respectively. By comparing the time characteristics and spectral characteristics of ultrafast fluorescence, it was found that the excitation energy of photosystem Ⅰ in P9 rice thylakoid membrane was faster than that of photosystem Ⅱ at grain filling or flowering stage; P9 and The excitation energy of SH 63 was higher than that of flowering at the filling stage. The spectral characteristics of the two thylakoid membranes were also presented. During the growth and development from flowering stage to filling stage , The pigment composition and binding status of the SH 63 thylakoid membrane changed, while P9 did not show this change.