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以不同的离子和不同的能量对7个品种的莲种子进行了注入,经过催芽和栽种,连续几年观察了对各离子注入组营养生长和生殖生长的诱变效应,并对其中一部分品种进行了RAPD分子检测。结果发现,离子注入的7个品种的表型均发生了不同情况的变异,包括株型、叶的直径、颜色、花冠数、花色、花期、雄蕊附属物颜色、雌蕊数目或种子的大小等。而且各个品种诱变产生的变异特征都经过3-5年以上能够比较稳定的遗传,已经形成了与各自对照有明显不同特点的突变体。RAPD分析表明,10个随机引物对各个突变体扩增结果显示:离子注入引起了莲的基因组发生变异,诱变产生的突变体出现了一些辐照敏感位点,主要表现为扩增片段的减少和增加,扩增片段分子量的变化,以及扩增谱带颜色深浅的变化。
Seven different varieties of lotus seeds were infused with different ions and different energies. After several years of inoculation and planting, the mutagenic effects on the vegetative and reproductive growth of ion implantation groups were observed, and some of them were subjected to RAPD molecular test. The results showed that there were different phenotypic changes in ion implanted species, including plant type, leaf diameter, color, number of corolla, flower color, flowering, stamen appendage color, pistil number or seed size. Moreover, the variation characteristics produced by each variety of mutagenesis can be more stable after more than 3-5 years of inheritance, and mutants having distinct characteristics with their respective controls have been formed. The results of RAPD analysis showed that the amplification results of 10 random primers on each mutant showed that ion implantation induced the genome variation of lotus, and some mutants induced by irradiation induced some radiation-sensitive sites, mainly showing the decrease of amplified fragment And increase the molecular weight of the amplified fragment changes, as well as the amplification of the color depth of the band changes.