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目的 建立一种操作简便、安全的核因子 κB(NF κB)活性检测方法。方法 采用夹心酶联免疫吸附测试法(SandwichELISA) ,酶标仪 ,验证NF κB活化的抑制剂反义白介素 1受体相关激酶 2 (IRAK 2 )寡核苷酸和N α Tosyl L lysinechloromethylketone(TLCK)对NF κB活性的抑制作用。结果 SandwichELISA测定结果表明 ,与对照组相比 ,用不同浓度反义IRAK 2寡核苷酸 (1,2 ,3,4μg )或TLCK(1,10 ,10 0 μmol·L-1)处理细胞后 ,NF κB活化受到不同程度的抑制 ,表现为吸光度值下降的幅度不同。结论 Sand wichELISA法可用于NF κB活性检测。
Objective To establish a simple and safe method for the detection of nuclear factor κB (NF κB) activity. Methods Antisense interleukin 1 receptor associated kinase 2 (IRAK 2) oligonucleotide and N α Tosyl L lysinechloromethylketone (TLCK) were detected by sandwich enzyme-linked immunosorbent assay (ELISA) and microplate reader. Inhibition of NF κB activity. Results Sandwich ELISA showed that compared with the control group, the cells were treated with different concentrations of antisense IRAK 2 oligonucleotide (1, 2, 3, 4 μg) or TLCK (1, 10, 10 μmol·L -1) , NF κB activation was inhibited to varying degrees, showing the magnitude of the decline in absorbance values. Conclusion Sand wich ELISA can be used to detect NF κB activity.