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由于丁型肝炎病毒(HDV)RNA能在缺乏乙型肝炎病毒(HBV)的培养细胞中复制,表明HBV唯一辅助功能是在HDV颗粒包装过程中,提供HBV外膜蛋白。为了证实这一结论,作者采用共同转染方法。作者采用多种质粒:pSVL(D_3)起动HDV复制,pSVL(Ag-S)表达S型HDV抗原,pSVL(Ag-L)表达L型HDV抗原,pSV2A-Neo(HBV)2表达HBV,pCMW82表达土拨鼠肝炎病毒(WHV),pSVL(WHVsAg)表达WHV小抗原。将质粒共同转染人肝癌细胞株,每3天收集1次培养液,总共培
Since hepatitis D virus (HDV) RNA can replicate in cultured cells lacking Hepatitis B virus (HBV), it is shown that the only helper function of HBV is to provide HBV outer membrane proteins during HDV particle packaging. To confirm this conclusion, the authors used a co-transfection method. The authors used a variety of plasmids: pSVL (D_3) initiated HDV replication, pSVL (Ag-S) expressed S HDV antigen, pSVL (Ag-L) expressed L-type HDV antigen, pSV2A-Neo (HBV) 2 expressed HBV and pCMW82 Woodchuck hepatitis virus (WHV), pSVL (WHVsAg) express WHV small antigen. The plasmid co-transfected human hepatocellular carcinoma cell line, collected once every 3 days culture medium, a total of training