论文部分内容阅读
目的：探讨胃泌素对人大肠癌增殖的调控作用。方法：体外细胞培养观察与肽胃泌素（ＰＧ）对人大肠癌ＳＷ４８０细胞株的影响，采用ＭＴＴ比色分析法测活癌细胞数，流式细胞术测ＤＮＡ和蛋白质合成及细胞增殖周期（ＰＩ）。结果：ＰＧ组（浓度２５ｍｇ／Ｌ）活细胞数，ＤＮＡ和蛋白质合成明显高于对照组（Ｐ＜０．０１），Ｇ０／Ｇ１期细胞数明显低于对照组（Ｐ＜０．０１），Ｓ、Ｇ２Ｍ期细胞数及ＰＩ明显高于对照组（Ｐ＜０．０１，Ｐ＜０．０５，Ｐ＜０．００１）。结论：胃泌素具有促进大肠癌细胞分裂和增殖的作用，在受体后参与了大肠癌细胞周期的调控作用。
Objective: To investigate the regulation of gastrin on the proliferation of colorectal cancer. Methods: The effects of peptide gastrin (PG) on the colorectal cancer SW480 cell line were observed in cell culture in vitro. The number of active cancer cells was measured by MTT colorimetric assay, DNA and protein synthesis and cell proliferation cycle were measured by flow cytometry ( PI). Results: The number of viable cells in the PG group (concentration 25 mg/L) was significantly higher than that in the control group (P<0.01), and the number of cells in the G0/G1 phase was significantly lower than that in the control group (P<0.01). The number of S and G2M cells and PI were significantly higher than those of the control group (P<0.01, P<0.05, P<0.001). CONCLUSION: Gastrin has the function of promoting the proliferation and proliferation of colorectal cancer cells, and participates in the regulation of the cell cycle of colorectal cancer after the recipient.