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目的:检测IGF-Ⅰ、IGF-ⅠR在结直肠癌及癌旁正常组织中蛋白水平的表达,分析其与临床病理学因素的关系,探讨其与结直肠癌的发生、浸润、分化、转移及预后的相关性,以便用于结直肠癌的早期诊断,预后判断及靶向治疗。方法:应用组织芯片及免疫组化技术,检测IGF-Ⅰ、IGF-ⅠR在结直肠癌组织、癌旁正常组织中蛋白水平的表达。结果:在结直肠癌组织中,IGF-I、IGF-ⅠR的蛋白水平阳性表达率分别为64.58%、58.33%,均显著高于癌旁正常组织中的16.67%、22.9%(P均为<0.05)。IGF-ⅠR表达水平与肿瘤的浸润程度、淋巴结转移、Dukes分期有关(P均为<0.05),而与其他病理因素无关(P>0.05),差异有统计学意义。结论:IGF-Ⅰ、IGF-ⅠR与结直肠癌的发生、发展中有关。IGF-ⅠR蛋白水平过表达与结直肠癌的浸润深度、Dukes分期有关(P<0.05),IGF-ⅠR对结直肠癌的早期诊断、预后判断及靶向治疗有重要意义。
OBJECTIVE: To detect the expression of IGF-Ⅰ and IGF-ⅠR in colorectal cancer tissues and adjacent normal tissues. The relationship between the expression of IGF-Ⅰ and IGF-ⅠR and the clinicopathological factors was analyzed, and its relationship with the occurrence, infiltration, differentiation and metastasis of colorectal cancer Prognosis of the correlation in order to be used for early diagnosis of colorectal cancer, prognosis and targeted therapy. Methods: The expression of IGF-Ⅰ and IGF-ⅠR in colorectal cancer tissues and adjacent normal tissues was detected by tissue microarray and immunohistochemistry. Results: The positive rates of IGF-I and IGF-ⅠR in colorectal cancer tissues were 64.58% and 58.33%, respectively, which were significantly higher than those in normal tissues (16.67% and 22.9%, P < 0.05). The expression of IGF-ⅠR was correlated with tumor infiltration, lymph node metastasis and Dukes stage (all P <0.05), but not with other pathological factors (P> 0.05). The difference was statistically significant. Conclusion: IGF-Ⅰ and IGF-ⅠR are associated with the occurrence and development of colorectal cancer. The overexpression of IGF-ⅠR was associated with the depth of invasion and Dukes stage (P <0.05), and IGF-ⅠR was of great importance in the early diagnosis, prognosis and targeted therapy of colorectal cancer.