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目的:探索操作方法简便、脱钙时间更短且对骨组织伤害较小的脱钙方法。方法:DBA/1小鼠40只,随机分组为胶原诱导关节炎模型组和正常组,并对模型组小鼠建立胶原诱导关节炎模型。分别取模型组和正常组踝关节,使用改良前后的脱钙方法进行脱钙,从关节病理及其评分、免疫组织化学方面进行比较脱钙效果。结果:经改良后,在脱钙温度4℃和脱钙液更换周期24 h条件下,脱钙速度较高(7 d),且此时对骨组织结构和骨组织内蛋白表达无明显影响。结论:优化的螯合剂脱钙法可以有效提高脱钙速度,可为快速制备病理诊断用骨切片提供了参考。
Objective: To explore a simple method of operation, decalcification time is shorter and less damage to the bone tissue of the decalcification method. Methods: Forty DBA / 1 mice were randomly divided into collagen-induced arthritis model group and normal group. Collagen-induced arthritis model was established in model group mice. The ankle joint of the model group and the normal group were taken, and the decalcification was performed by using the decalcification method before and after the improvement. The decalcification effect was compared on the basis of the joint pathology and the score and the immunohistochemistry. RESULTS: After the decalcification temperature was 4 ℃ and the decalcification solution replacement cycle was 24 h, the decalcification speed was higher (7 d), and there was no significant effect on the bone tissue structure and protein expression in the bone tissue. Conclusion: Optimized chelator decalcification method can effectively increase the rate of decalcification, which can provide a reference for rapid preparation of pathological diagnosis of bone slices.