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目的建立肝郁脾虚型肠易激综合征(IBS)模型组和对照组结肠组织蛋白质双向电泳图谱,结合质谱技术鉴定并分析差异表达蛋白,对候选表达差异蛋白进行蛋白质功能和相关研究,进一步阐明肝郁脾虚型IBS病理机制。方法采用双向凝胶电泳法分离IBS模型组和正常对照组结肠粘膜差异蛋白,运用MALDI TOF/TOF串联飞行时间质谱仪进行鉴定,运用Gene Ontology和Pathway Studio生物信息学方法将所鉴定的差异蛋白进行功能分类,分析IBS病理机制中关键蛋白。结果首次运用蛋白组学的方法寻找IBS模型组和正常对照组结肠的差异表达蛋白特征谱,结果发现差异倍数在2.5倍以上的差异蛋白有15个,其中2个蛋白表达上调,13个蛋白表达下调。结论热休克蛋白、硫氧还蛋白、氯离子通道蛋白等可能是肝郁脾虚型IBS病理机制中潜在分子标志物。
Objective To establish a two-dimensional gel electrophoresis pattern of colon tissue in IBS model group and control group with liver-qi stagnation and spleen-deficiency syndrome, identify and analyze differentially expressed proteins by mass spectrometry and further elucidate the protein function of the differentially expressed protein candidates Pathological mechanism of IBS with liver depression and spleen deficiency syndrome. Methods Two-dimensional gel electrophoresis was used to separate colonic mucosal proteins from IBS model group and normal control group. MALDI TOF / TOF tandem time-of-flight mass spectrometry was used to identify the differential proteins identified by Gene Ontology and Pathway Studio bioinformatics Functional classification, analysis of IBS pathogenesis of key proteins. Results For the first time using proteomics method to find differential expression protein profiles in IBS model group and normal control group, there were 15 differential proteins with more than 2.5 fold difference, of which 2 were up-regulated and 13 were expressed Down. Conclusions Heat shock protein, thioredoxin and chloride ion channel protein may be potential molecular markers in the pathogenesis of IBS with liver depression and spleen deficiency syndrome.