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目的 研究缺氧对小鼠瘦素及其受体 (包括转运性受体Ra、Rc和功能性受体Rb)mRNA表达变化的影响,以进一步明确瘦素和呼吸功能的关系。方法 利用医学自动测控缺氧仓(XQ Ⅰ型)复制机体常压缺氧状态,将小鼠分为正常对照组、缺氧 24h组、缺氧 48h组,然后利用逆转录 聚合酶链反应(RT-PCR)分别定量检测瘦素及其受体mRNA表达水平。根据cDNA条带的灰度值计算其相对含量,各种产物与磷酸甘油醛脱氢酶 (GADPH)的比值表示其相对表达水平。结果(1)缺氧 24h组和缺氧 48h组瘦素mRNA表达水平分别为 0.903±0.190、0.856±0.336,显著高于正常对照组(0.508±0.207,P均<0.05); (2)缺氧 24h组和缺氧 48h组RamRNA表达水平分别为0 724、0.700,分别为正常对照组 (0 630)的 115%、111%; (3)缺氧 24h组和缺氧 48h组RbmRNA表达水平分别为 0.381±0.038、0.345±0.042,与正常对照组(0.258±0.049)比较差异均有统计学意义(P均<0.05); (4)缺氧 24h组和缺氧 48h组RcmRNA表达水平分别为 0.299、0.292,分别为正常对照组(0.133)表达水平的 224%、219%。结论 缺氧作为一种独立因素可在一定范围内刺激机体瘦素、瘦素受体Ra、Rb、Rc基因表达增加,从而在正向调节机体呼吸功能中发挥重要作用。
Objective To study the effect of hypoxia on the mRNA expression of leptin and its receptors (including transporter receptors Rc, Rc and functional receptor Rb) in mice to further clarify the relationship between leptin and respiratory function. Methods The mice were randomly divided into normal control group, hypoxia 24h group, hypoxia 48h group, and then were detected by reverse transcriptase-polymerase chain reaction (RT) -PCR) were used to detect the mRNA expression of leptin and its receptor respectively. According to the gray value of cDNA bands, the relative content of these products was calculated. The ratio of various products to glyceraldehyde phosphate dehydrogenase (GADPH) expressed their relative expression levels. Results (1) The levels of leptin mRNA in hypoxia 24h group and hypoxia 48h group were 0.903 ± 0.190 and 0.856 ± 0.336, respectively, which were significantly higher than those in control group (0.508 ± 0.207, P <0.05). (2) The mRNA expression levels of RamRNA in 24 hours and 48 hours hypoxia group were 0 724 and 0.700, respectively, which were 115% and 111% of the normal control group (0 630) respectively. (3) The mRNA expression levels of RbmRNA in hypoxia 24h group and hypoxia 48h group were 0.381 ± 0.038and0.345 ± 0.042, respectively, which were significantly different from the normal control group (P <0.05). (4) The expression of RcmRNA in hypoxic 24h group and hypoxia 48h group were 0.299, 0.292, respectively, the normal control group (0.133) 224%, 219%. Conclusion As an independent factor, hypoxia can stimulate leptin in a certain range. The expressions of Ra, Rb and Rc in leptin receptor are increased, which play an important role in the positive regulation of respiratory function.