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应用红四唑、蓝四唑和新四唑测定了7个科共23个种农作物和饲料牧草种子的生活力,结果比较准确可靠。四唑法测定大豆及几种豆科牧草休眠种子的生活力,快速准确,远比常规法优越。红四哇易透过种皮及胚组织,蓝四唑反应灵敏,故染色速度均快,而新四唑相反,染色速度慢。红四唑易在胚组织或种子切口表面形成白色沉淀物和不易使多种油料种子胚根染色,特别是在被处理种子与空气充分接触时更为明显。故在应用红四唑时应适当加多药液用量,使种子处在液面下较深处,或改变一般沿用的种子预处理方法,将种子吸湿软化后不进行露胚而直接染色,或只切破种皮再染色,使种子胚部不接触或少接触空气,可提高染色效果,以利判断。新四唑能使多种油料种子胚根和子叶充分染色,在胚组织表面形成白色沉淀物的现象也较轻微,对露胚种子的染色效果很好,但对某些不便露胚的小粒种子,则因不易透过种皮,染色速度过慢,效果不好。试验了在30℃和40℃下用三种四唑盐溶液处理种子,各自需要的染色时间,可供进行四唑测定时参考。
The viability of 23 species of crops and forages in 7 families was determined using red tetrazolium, blue tetrazole and neotetrazole. The results were more accurate and reliable. The tetrazolium method for determining the viability of dormant seeds of soybean and several leguminous pastures is fast and accurate and far superior to conventional methods. Red Si Wow easily through the seed coat and embryo tissue, blue tetrazole sensitive, so fast staining speed, while the new tetrazolium contrast, slow staining. Red tetrazolium easily formed embryo tissue or seed incision surface white precipitate and not easy to make a variety of oil seed radicle staining, especially in dealing with seeds and air full contact more obvious. Therefore, the application of red tetrazolium should be appropriate to increase the amount of liquid, so that the seeds deep in the liquid surface, or change the general use of seed pretreatment methods, the seeds absorb moisture softening without exposed embryos and direct staining, or Only cut the seed coat re-staining, so that the seed embryo does not touch or less contact with air, can improve the staining effect, to facilitate judgment. Neotetrazole can make a variety of oil seed radicle and cotyledons full staining, the formation of white precipitate on the surface of the embryo is also a minor phenomenon, the embryo germ staining is very good, but for some inconvenience of embryo germination of small seeds , Due to difficult to penetrate the seed coat, dyeing speed is too slow, the effect is not good. The seeds treated with the three tetrazolium salt solutions at 30 ° C and 40 ° C were tested for their respective required staining times for reference when the tetrazolium assay was conducted.