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目的:了解瓯海区食源性金黄色葡萄球菌流行特点及其肠毒素和耐药性,为防治金黄色葡萄球菌引起的食源性疾病提供科学依据。方法:参照GB/T4789.10-2003对采集的冷菜和熟肉制品样本进行金黄色葡萄球菌分离、生化鉴定和药物敏感试验,同时利用PCR方法和mini VIDAS方法对金黄色葡萄球菌进行肠毒素携带和分型分布的分析。结果:从177件样品中检出45株金黄色葡萄球菌,其中产肠毒素和肠毒素基因阳性32株,其肠毒素阳性率达71.11%。结论:瓯海区冷菜和熟肉制品等食品存在着较严重的金黄色葡萄球菌潜在污染,其产肠毒素能力较强,以A型肠毒素为主,其对多种药物的耐药性提示适当选用药物的重要性。
Objective: To understand the epidemic characteristics of Staphylococcus aureus and its enterotoxin and drug resistance in Ouhai District, and to provide a scientific basis for the prevention and treatment of foodborne diseases caused by Staphylococcus aureus. Methods: According to GB / T4789.10-2003, the samples of cold vegetables and cooked meat samples were separated, biochemically identified and susceptible to S. aureus. Meanwhile, PCR and mini VIDAS were used to detect Staphylococcus aureus enterotoxin Carry and type distribution analysis. Results: Staphylococcus aureus were detected in 177 samples, of which 32 were positive for enterotoxigenic and enterotoxin genes. The positive rate of enterotoxin was 71.11%. CONCLUSION: There is a serious potential contamination of Staphylococcus aureus in foods such as cold-pickled vegetables and cooked meat products in Ouhai District. The production of enterotoxins is strong, with type A enterotoxins predominant and its resistance to various drugs Prompt selection of the importance of the drug.