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目的 :观察急性呼吸窘迫综合征 (ARDS)合并左心功能降低时血浆和心肌组织脂质过氧化和一氧化氮(NO)的动态变化 ,以了解它们在左心功能降低中的作用。方法 :用油酸缓慢 (iv:耳缘静脉 )注射诱发兔肺损伤所致ARDS模型并分为 30 min、6 0 min、90 min和 12 0 min4组。结果 :(1)油酸各时间组血浆和心肌组织的 GSH- Px和MDA含量与对照组相比有显著差异 (P <0 .0 5～ 0 .0 1) ,而且二者呈负相关 (r =- 0 .98,P <0 .0 1) ;(2 )油酸 30min和 6 0 min组血浆 NO含量高于对照组 (P <0 .0 1) ,在油酸 90 min和 12 0 min组比油酸 6 0 min组又有显著减少 (P <0 .0 1) ;(3)心肌组织 NO含量在油酸 30 min时比对照组明显增加 (P <0 .0 1) ,而在油酸 6 0 min以后各组与油酸 30 min组相比又有所减少 (P <0 .0 1) ;(4 )电镜下见到心肌细胞结构有明显改变。 结论 :油酸致 ARDS过程左心功能降低与脂质过氧化和 NO增加有关
OBJECTIVE: To observe the dynamic changes of lipid peroxidation and nitric oxide (NO) in plasma and myocardial tissue in patients with acute respiratory distress syndrome (ARDS) combined with decreased left ventricular function, so as to understand their role in the reduction of left ventricular function. Methods: The acute lung injury induced ARDS models were induced by oleic acid slowly (iv: auricle vein) and divided into 30 min, 60 min, 90 min and 120 min4 groups. Results: (1) The contents of GSH-Px and MDA in plasma and myocardium of oleic acid group were significantly different from those in control group at each time point (P <0.05-0.01), and both were negatively correlated r = - 0.98, P <0.01); (2) The contents of NO in plasma at 30 min and 60 min of oleic acid were higher than that of the control group (P <0.01) (P <0.01). (3) The content of NO in myocardium increased significantly (P <0.01) at 30 min in oleic acid group compared with the control group After 60 min of oleic acid, the number of oleic acid decreased again compared with 30 min group (P <0.01). (4) The changes of myocardial cell structure were observed under electron microscope. CONCLUSION: Decreased left ventricular function in oleic acid-induced ARDS is associated with increased lipid peroxidation and NO