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1985年,我们通过人工培养陆地棉茎尖取得再生植株。近二年,在改进培养基后,取得了两方面的进展:1、再生苗基部产生大量胚性愈伤组织,转入分化培养基后,分化出大量再生植株;2、茎节腋间产生不定芽,形成多苗或丛芽。并取得一次再生完整植株或分茎叶和生根二阶段产生植株。发现基础培养基SJ-1附加2,4-D和2ip或KIN,葡萄糖或蔗糖,出现绿芽(头)频率较高于MS培养基。研究结果指出,陆地棉茎尖人工培养诱导批量成苗,要求营养和激素条件较宽,愈伤组织再生效率高,一个茎尖经2个月培养可产生5个苗。采用了海岛棉杂种茎尖培养,结果相似。
In 1985, we obtained regenerated plants by artificially cultivating the tips of upland cotton. In the past two years, two progresses have been made after the improvement of medium: 1, a large amount of embryogenic callus is produced on the base of regenerated seedlings, and a large number of regenerated plants are differentiated after being transformed into differentiation medium; 2, Adventitious buds, the formation of more shoots or buds. And obtain a complete regeneration of plants or divided stems and leaves second stage rooting plants. Found that the basic medium SJ-1 added 2,4-D and 2ip or KIN, glucose or sucrose, the emergence of green shoots (head) frequency higher than the MS medium. The results indicated that the in vitro culture of shoot tip of G. australis induces batch seedlings, which requires wide nutrition and hormone conditions and high callus regeneration efficiency. Five shoots can be produced after one shoot tip is cultured for 2 months. Using island cotton hybrid shoot tip culture, the results are similar.