宁夏枸杞胚珠孚尔根染色后经透明用激光扫描共聚焦显微镜直接观察各发育时期胚珠内部结构。结果显示,用孚尔根染色后,枸杞大孢子发生和雌配子体发育的各个阶段都可在激光共聚焦显微镜下清楚呈现。此种方法克服了胚囊因深埋在胚珠体细胞组织中而难以观察的问题。与经典的切片方法相比,该法可对胚珠整体进行观察,操作简单、可在较短时间内大规模地检测胚囊发育状况。 The Lycium barbarum root of Lycium barbarum was stained with transparent laser scanning confocal microscopy to observe the internal structure of ovules at various developmental stages. The results showed that each stage of Lycium barbarum sporulation and female gametophyte development could be clearly visualized by laser scanning confocal microscopy after staining with Fork root. This method overcomes the problem that the embryo sac is hard to observe due to its deep burial in ovule cells. Compared with the classical slicing method, this method can observe the ovule as a whole and has simple operation and can detect the embryo sac development in large scale in a short time.