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目的:建立检测HSF1 mRNA的real-time PCR的方法,了解肝细胞癌患者外周血中HSF1的表达水平及其与各临床病理特征之间的关系。方法:利用real-time PCR的方法检测20例肝细胞癌患者及20例正常人群外周血中HSF1 mRNA的表达量。结果:肝细胞癌患者外周血中的HSF1 mRNA表达量显著高于正常人群(P<0.05);肝细胞癌患者外周血中HSF1 mRNA的表达水平在不同性别、肿瘤大小、门静脉侵犯情况、HbsAg水平及AFP水平的患者中的差异无统计学意义(P>0.05);在不同病理分化程度、TNM分期的患者中的差异有统计学意义(P<0.05)。结论:real-time PCR技术可以成功检测外周血中HSF1 mRNA的表达量,HSF1可能与肝细胞癌的发生发展密切相关。
OBJECTIVE: To establish a real-time PCR method for detecting HSF1 mRNA in peripheral blood of patients with hepatocellular carcinoma to find out the relationship between HSF1 expression and clinicopathological features. Methods: Real-time PCR was used to detect the expression of HSF1 mRNA in 20 patients with hepatocellular carcinoma and 20 normal controls. Results: The expression of HSF1 mRNA in peripheral blood of patients with hepatocellular carcinoma was significantly higher than that of normal people (P <0.05). The expression of HSF1 mRNA in peripheral blood of patients with hepatocellular carcinoma was significantly higher than that of controls (P> 0.05). There was significant difference in patients with different histological grade and TNM stage (P <0.05). Conclusion: Real-time PCR can successfully detect the expression of HSF1 mRNA in peripheral blood. HSF1 may be closely related to the occurrence and development of hepatocellular carcinoma.