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陆地棉和海岛棉是两个不同的四倍体栽培种 ,但在生产上各有其特点 ,陆地棉丰产性强 ,海岛棉纤维品质优良 ,利用其种间杂交群体定位产量和品质性状的QTL ,对于分子标记辅助的海岛棉优质纤维向陆地棉转移很有意义。以SSR和RAPD为分子标记 ,陆地棉与海岛棉杂种 (邯郸 2 0 8×Pima90 )F2 群体为作图群体 ,构建了一张含 12 6个标记的遗传图谱 ,包括 6 8个SSR标记和 5 8个RAPD标记 ,可分为 2 9个连锁群 ,标记间平均距离为 13 7cM ,总长1717 0cM ,覆盖棉花总基因组约 34 34% ;以遗传图 12 6个标记为基础 ,对F2 :3 家系符合正态分布的 10个农艺性状及纤维品质性状进行全基因组QTL扫描 ,结果发现 2 9个QTL分别与产量和品质性状有关。其中与衣指、籽指、皮棉产量、子棉产量、衣分等产量性状相关的QTL分别有 1、3、5、6和 1个 ,与纤维长度、整齐度、强度、伸长率和马克隆值等品质性状相关的QTL分别有 2、4、2、4和 1个。各QTL解释的变异量在 12 4 2 %～ 47 0 1%之间。其中比强度有关的 2个QTL能够解释的表型变异率分别为 34 15 %和 13 86 %。
Gossypium hirsutum and Gossypium barbadense were two different tetraploid cultivars, but they all had their own characteristics in production. The cotton cultivars with high yield and high quality on G. gossypii were used as QTLs for yield and quality traits in interspecific hybrids , Which is of great significance for the transfer of molecular marker-assisted highland fiber to upland cotton. Using SSR and RAPD markers as markers, F2 population of G. hirsutum × G. barbadense hybrids (Handan 208 × Pima90) was used as a mapping population to construct a genetic map containing 12 6 markers, including 68 SSR markers and 5 The 8 RAPD markers could be divided into 29 linkage groups, with an average distance of 13 7 cM and a total length of 1717 0 cM, covering 34 34% of the total cotton genome. Based on 12 6 markers of genetic map, Genome-wide QTLs of 10 agronomic traits and fiber quality traits which were in normal distribution were found. The results showed that 29 QTLs were related to yield and quality traits respectively. The QTLs related to yield traits such as finger, seed index, lint yield, seed cotton yield and lint yield were 1, 3, 5, 6 and 1, respectively, which were related to the length, uniformity, There were 2, 4, 2, 4 and 1 QTL related to quality traits such as clonal value. The amount of variation explained by each QTL ranged from 12 4 2% to 47 0 1%. Among them, two QTLs related to intensity could explain phenotypic variation rates of 34 15% and 13 86% respectively.