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讨论稻子、小麦麸皮中可溶性非淀粉多糖的气相色谱法测定。将稻子、小麦麸皮磨细后,用80%乙醇萃取游离糖,剩余物在醋酸缓冲液(pH5.0)条件下分别用α-淀粉酶和α-葡萄糖苷酶酶解,除掉淀粉。非淀粉多糖用酸水解为各单糖,采用糖醇衍生化成乙酸酯的制备方法,用OV-1701石英毛细管色谱柱,可分离得到非淀粉多糖(NSP)各组成单糖的色谱峰。并以自然界中不存在的阿洛糖作内标物,提高了定性定量分析的准确性,计算出了稻子、小麦麸皮中可溶性非淀粉多糖的含量。
Discussion on Determination of Soluble Non-Starch Polysaccharides in Rice and Wheat Bran by Gas Chromatography. After the rice bran and wheat bran were ground, the free sugars were extracted with 80% ethanol. The residue was enzymatically decomposed with α-amylase and α-glucosidase in acetic acid buffer (pH 5.0) to remove the starch. Non-starch polysaccharides were acid-hydrolyzed into monosaccharides, and the derivatization of sugar alcohols to form acetates was performed. The chromatographic peaks of monosaccharides of non-starch polysaccharides (NSP) were separated by OV-1701 quartz capillary column. In addition, Arose sugar, which is not found in nature, was used as an internal standard to improve the accuracy of qualitative and quantitative analysis. The contents of soluble non-starch polysaccharides in rice and wheat bran were calculated.