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目的 观察小鼠着床前的胚胎细胞中原癌基因ras编码的蛋白产物 p2 1Ras及c erbB2的表达 ,并了解其对着床前的胚胎发育的可能作用。方法 采用免疫组织化学ABC法 ,检测着床前小鼠胚胎中p2 1Ras及c erbB2蛋白的表达。结果 小鼠着床前的胚胎细胞中 p2 1Ras的免疫反应呈阳性 ,阳性物质分布于胞质中 ,胚胎外透明带呈阴性反应 ;而在各期着床前的胚胎中均未检测到c erbB2蛋白的表达。结论 小鼠着床前的胚胎中 ,有原癌基因ras编码蛋白的表达 ,p2 1Ras是增殖和分化受体介导的信号传导途径中的重要组成部分 ,可能在小鼠着床前的胚胎发育过程中起重要的调节作用。C erbB2基因对于围着床期子宫的生长发育具有重要的作用 ;而在小鼠着床前的胚胎发育过程中似乎不起重要作用
Objective To observe the expression of ras-encoded protein products p2 1Ras and c erbB2 in preimplantation embryo cells and to investigate the possible effect on preimplantation embryo development. Methods Immunohistochemical ABC method was used to detect the expression of p2 1 Ras and c erbB2 in preimplantation mouse embryos. Results The immunoreactivity of p2 1 Ras in preimplantation embryos was positive. The positive substances were distributed in the cytoplasm and the extraembryonic zona pellucida was negative. However, c erbB2 was not detected in preimplantation embryos Protein expression. Conclusion The expression of ras protein in the preimplantation mouse embryo is an important part of the signal transduction pathways mediated by proliferation and differentiation receptors and may play an important role in preimplantation mouse embryo development Play an important regulatory role in the process. The C erbB2 gene plays an important role in the growth and development of the uterus around the bed; however, it seems to play an important role in embryo development before implantation in mice