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本文选择已生育子女的25～35岁正常男子的精液标本,分成正常对照及分别经洗必泰(2mg/ml)、NP-9(0.25mg/ml)、MN-863(0.2mg/ml)、Ⅲ号原药(0.05mg/ml)和复方Ⅰ号(0.1mg/ml)处理的六组。用组织化学、免疫组织化学、荧光染色等方法分别显示精子DNA、RNA、磷脂、糖类,SDH、LDH、AP、ATPase、hCG、IgM、Y小体等成分,比较用药前后的变化,从而评价上述几种外用杀精剂的作用与机制。实验结果表明此组外用杀精剂不损害人的精子细胞核及DNA、RNA、磷脂以及Y小体的形态。洗必泰可破坏精子质膜的脂糖蛋白,明显干扰精子的多种酶活性;MN-863、Ⅲ号原药、复方Ⅰ号可损害精子质膜功能,干扰用能酶的活性;NP-9可损伤精子质膜的脂糖蛋白,干扰其用能与供能酶的活性。这组人精子细胞化学定位的方法,可有效地检测人精子的缺陷与损伤。
In this study, semen samples of 25-35-year-old normal children who had children were divided into normal control group and MN-863 (0.2mg / ml), chlorhexidine (2mg / ml) , Ⅲ original drug (0.05mg / ml) and compound Ⅰ (0.1mg / ml) treated six groups. The changes of sperm DNA, RNA, phospholipids, carbohydrates, SDH, LDH, AP, ATPase, hCG, IgM and Y bodies were observed by histochemistry, immunohistochemistry and fluorescence staining before and after treatment, The above several topical spermicide role and mechanism. The experimental results show that this group of topical spermicide does not harm the human sperm nucleus and DNA, RNA, phospholipids and Y body morphology. Chlorhexidine destroys sperm plasmalemma lipoprotein, and obviously interferes with many enzyme activities of sperm; MN-863, Ⅲ original drug, compound Ⅰ can damage sperm plasma membrane function, interfere with the activity of enzyme; NP- 9 can damage the sperm membrane lipoprotein, interfere with its energy and energy-feeding enzyme activity. This group of human sperm cell chemical localization method can effectively detect human sperm defects and injuries.