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目的探讨妊娠期二月桂酸二丁基锡(DBTD)暴露对子代雄性大鼠性成熟后生殖系统的影响及其作用机制。方法将16只健康SPF级妊娠Wistar大鼠随机分为4组,分别为溶剂对照(玉米油)组和低(10 mg/kg)、中(20 mg/kg)、高剂量(30 mg/kg)DBTD染毒组,每组4只。采用灌胃方式进行染毒,染毒容量为5 ml/kg,自妊娠第12～20天连续染毒。仔鼠出生后第70天,每组随机抽取10只雄性大鼠,称重后处死,测定睾丸和附睾重量及其脏器系数、附睾精子数以及血清中黄体生成素(LH)、卵泡刺激素(FSH)和睾酮(T)以及睾丸组织中睾酮(T)的水平,并观察睾丸组织病理学改变。结果各剂量DBTD染毒组子代雄性大鼠体重、附睾重量及其脏器系数、血清中LH和FSH水平与溶剂对照组相比,差异均无统计学意义。与溶剂对照组比较,高剂量DBTD染毒组子代雄性大鼠睾丸重量及其脏器系数,中、高剂量DBTD染毒组子代雄性大鼠附睾精子数较高,差异均有统计学意义(P<0.05或P<0.01)。且随着DBTD染毒剂量的升高,子代雄性大鼠睾丸重量及其脏器系数以及附睾精子数均呈升高趋势。与溶剂对照组比较,高剂量DBTD染毒组子代雄性大鼠血清T水平和各剂量DBTD染毒组子代雄性大鼠睾丸T水平均较高,差异有统计学意义(P<0.05或P<0.01)。结论在本实验染毒时间和剂量范围内,孕期DBTD染毒可干扰子代雄性大鼠体内T的合成和代谢,从而促进睾丸发育和精子形成。
Objective To investigate the effects of dibutyltin dilaurate (DBTD) exposure on the reproductive system of sexual maturity in offspring male rats and its mechanism. Methods Twenty-six healthy Wistar pregnant rats of SPF grade were randomly divided into four groups: control group (corn oil) and low (10 mg / kg), medium (20 mg / kg) ) DBTD exposure group, 4 rats in each group. Gavage by way of exposure, exposure capacity of 5 ml / kg, from the first 12 to 20 days of pregnancy continuous exposure. On the 70th day after birth, 10 male rats were randomly selected from each group and weighed and killed. The weight of testis and epididymis and their organ coefficients, the number of epididymal sperm, serum LH, FSH, (FSH) and testosterone (T), testosterone (T) levels in testis and testicular histopathological changes. Results Body weight, epididymis weight and organ coefficient, serum LH and FSH levels in offspring of DBTD-treated mice were not significantly different from those of the solvent control group. Compared with the solvent control group, the testicular weight, the organ coefficient, the number of epididymal sperm in the male rats with high dose DBTD exposure and the high dose DBTD exposure group were significantly higher (P <0.05 or P <0.01). And with the DBTD dose increased, testicular weight and organ coefficient of offspring male rats and the number of epididymal sperm showed an upward trend. Compared with the solvent control group, the serum T level of the male rats in the high dose DBTD group and the testicular T level in the male rats in each dose of DBTD exposure group were significantly higher than those in the control group (P <0.05 or P <0.01). Conclusion DBTD exposure during pregnancy can interfere with the synthesis and metabolism of T in the offsprings of male offspring in order to promote testicular development and spermatogenesis.