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旨在初步探讨丛枝菌根真菌GV对丹参木质结构及合成相关基因PAL、C4H,防御相关基因PR1、WRKY的影响。实验设定对照组和菌根组,测定生长90天后菌根对丹参生长情况及木质部结构的影响,同时采用实时荧光定量的方法测定相关基因PAL、C4H及30、60、75、90、105、130天不同时期PR1、WRKY的表达情况。接种菌根90天丹参根部侵染率为90%,基本达到最大值;地上部分鲜重和叶片数显著高于对照组,约为对照组的2.7倍和1.96倍;根部横切部分的木质部面积,积分光密度显著大于对照组;与对照组相比,地下部分木质素合成基因PAL、C4H表达显著提高,地上部分显著降低;丹参防御基因PR1(地下部分)和WRKY表达量在75天前显著增加,地下部分WRKY表达量在130天显著增加。丛枝菌根真菌能促进丹参生长,增加丹参根部木质部面积以及密度,促进木质素的合成,对提高丹参抗病性有积极作用。
The aim of this study is to investigate the effects of arbuscular mycorrhizal fungi GV on the woody structure and the genes related to PAL, C4H, defense-related genes PR1 and WRKY. The control group and mycorrhizal group were set up and the mycorrhizal effect on the growth of Salvia miltiorrhiza and the structure of xylem were measured 90 days after the growth. The relative gene PAL, C4H and 30,60,75,90,105 were detected by real- The expression of PR1 and WRKY in different period of 130 days. Inoculation mycorrhizal root 90 days Salvia root infection rate was 90%, basically reached the maximum value; aboveground fresh weight and leaf number was significantly higher than the control group, about 2.7 times and 1.96 times the control group; root cross section xylem area , And the integral optical density was significantly greater than that of the control group. Compared with the control group, the expression of PAL and C4H in the underground lignin gene was significantly increased and the aerial part was significantly decreased. The expression levels of PR1 (underground part) and WRKY in Danshen resistance gene were significant 75 days before Increasing, the underground WRKY expression increased significantly at 130 days. Arbuscular mycorrhizal fungi can promote the growth of Salvia miltiorrhiza, increase the root area and density of Salvia miltiorrhiza, promote the synthesis of lignin, and improve the disease resistance of Salvia miltiorrhiza.