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目的:研究老龄大鼠急性脑缺血再灌注对心肌组织的影响。方法:参照Zea Longa线栓法建立老龄大鼠局灶性脑缺血再灌注模型,从心肌酶谱、心电图及心肌超微结构等方面观察对心脏的影响。结果:与对照组比,在脑缺血2 h再灌注3和6 h组AST和LDH升高明显;CPK和CPK-MB在3、6和12 h组均有显著性升高(t检验,P<0.05)。大脑中动脉阻塞后出现ECG异常发生率为53.13%,6、12 h组明显高于32、4 h组(χ2检验,P<0.05),主要表现为S-T段的上抬和各种心律失常,在脑缺血30 min即可出现,70.59%发生在脑缺血后2 h和再灌注6 h内。心肌超微结构的改变在脑缺血2 h再灌注3和6 h组最明显,表现心肌缺血性损害和胞质中糖原颗粒明显减少。结论:急性脑缺血及再灌注后对心肌有明显损伤作用,以脑缺血2 h和再灌注6 h内最明显。
Objective: To study the effect of acute cerebral ischemia-reperfusion on myocardial tissue in aged rats. Methods: The model of focal cerebral ischemia-reperfusion in aged rats was established according to the Zea Longa method. The effects on the heart were observed from myocardial zymogram, electrocardiogram and myocardial ultrastructure. Results: Compared with the control group, the levels of AST and LDH increased significantly at 3 h and 6 h after reperfusion at 2 h. The CPK and CPK-MB increased significantly at 3, 6 and 12 h (t test, P <0.05). The incidence of ECG abnormalities after occlusion of the middle cerebral artery was 53.13%, significantly higher than that in the 6 and 12 h groups (χ2 test, P <0.05). The main manifestations were ST elevation and various arrhythmias, In 30 minutes after cerebral ischemia can occur, 70.59% occurred in 2 h after cerebral ischemia and reperfusion within 6 h. Myocardial ultrastructure changes in the ischemia 2 h reperfusion 3 and 6 h group most obvious myocardial ischemic damage and cytoplasmic glycogen granules was significantly reduced. CONCLUSION: Myocardial injury after acute cerebral ischemia and reperfusion is obvious, which is most obvious in 2 h of cerebral ischemia and 6 h of reperfusion.