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目的探究炎症调节基因miR-155和miR-146a及炎症相关基因在小鼠血吸虫病及吡喹酮治疗中的表达特征,为进一步阐明吡喹酮治疗血吸虫病的作用机制奠定基础。方法以BALB/c小鼠为研究对象,建立日本血吸虫感染的动物模型;小鼠随机分为4组:正常组、感染6周组、感染12周组和吡喹酮(PZQ,300 mg/kg 1次灌胃杀虫治疗)治疗组。以HE染色观察肝脏病理变化;以Real-time PCR检测肝脏miR-155和miR-146a及炎症相关基因TNF-α、IL-1β和IL-6的mRNA水平。结果感染6周组小鼠的肝脏miR-155、miR-146a及TNF-α、IL-1β和IL-6的mRNA水平均显著高于正常组和感染12周组(P<0.05);与感染12周组小鼠相比,PZQ治疗组小鼠肝脏虫卵肉芽肿反应减轻,且肝脏miR-155、miR-146a及TNF-α、IL-1β和IL-6的mRNA水平有所升高(P<0.05)。结论本研究发现miR-155和miR-146a可能与血吸虫病肝脏炎症的发生发展有关,并且参与了吡喹酮对炎症的调节。
Objective To investigate the expression of inflammatory regulatory genes miR-155 and miR-146a and inflammation related genes in mouse schistosomiasis and praziquantel treatment, and lay a foundation for further clarifying the mechanism of action of praziquantel in treating schistosomiasis. Methods The BALB / c mice were used as the research object to establish an animal model of Schistosoma japonicum infection. The mice were randomly divided into 4 groups: normal group, 6-week infected group, 12-week infected group and praziquantel (PZQ, 300 mg / kg 1 gavage insecticide treatment) treatment group. Liver histopathological changes were observed by HE staining. The mRNA levels of miR-155 and miR-146a and TNF-α, IL-1β and IL-6 in liver were detected by Real-time PCR. Results The mRNA levels of miR-155, miR-146a, TNF-α, IL-1β and IL-6 in the liver of infected mice at 6th week were significantly higher than those of normal group and 12th week of infection (P <0.05) Compared with the 12-week-old mice, the liver granuloma reaction in PZQ-treated mice was reduced and the levels of mRNA of miR-155, miR-146a and TNF-α, IL-1β and IL- P <0.05). Conclusion This study found that miR-155 and miR-146a may be involved in the development of liver inflammation in schistosomiasis and is involved in the regulation of inflammation by praziquantel.