一、体外培养的成人食管粘膜组织块不但可以长出许多生长旺盛的上皮细胞,还可经多次迁移接种培养,不断提供大量新的上皮细胞。组织块的体外存活时间最长可达4个月以上。二、低钙、低血清加激素及生长因子等成份的199培养液,可以促进上皮细胞从组织块迁移出来,并抑制成纤维细胞的生长。三、本实验体系为进行食管上皮细胞癌变的研究,提供了一个较好的体外实验系统。 First, in vitro cultured adult esophageal mucosa not only can grow a lot of growing epithelial cells, but also after repeated inoculation culture, continue to provide a large number of new epithelial cells. Tissue blocks survive in vitro up to 4 months or more. Second, low calcium, low serum plus hormone and growth factor and other ingredients of 199 culture medium, can promote the migration of epithelial cells from the tissue block, and inhibit the growth of fibroblasts. Third, the experimental system for the study of esophageal epithelial cell carcinoma, provides a better in vitro experimental system.