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目的建立同时测定伤科七味片中士的宁和马钱子碱含量的方法。方法采用Agilent ZORBAXSBC18柱(4.6mm×250mm,5μm),流动相:乙腈-0.4%磷酸水溶液(用三乙胺调节pH值为3.50)(10∶90),流速1.0mL·min-1,检测波长:254nm。结果士的宁和马钱子碱的线性范围分别为0.0096～0.0960g·L-1,0.0049～0.0490g·L-1;平均加样回收率(n=6)分别为0.38%,0.74%。结论该方法简便快速、结果准确可靠,适用于伤科七味片的质量控制。
Objective To establish a method for the simultaneous determination of strychnine and strychnine in Shangke Qiwei Tablets. Methods Agilent ZORBAXSBC18 column (4.6mm×250mm, 5μm) was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid aqueous solution (pH adjusted to 3.50 with triethylamine) (10∶90). The flow rate was 1.0mL·min-1. : 254 nm. Results The linear ranges of strychnine and strychnine were 0.0096-0.0960 g·L-1 and 0.0049-0.0490 g·L-1, respectively. The average recovery (n=6) was 0.38% and 0.74%, respectively. Conclusion The method is simple and rapid, and the result is accurate and reliable. It is suitable for the quality control of traumatic Qiqi tablets.