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The 930 bp segment in 3’ terminal region of Strawberry mild yellow edge virus(SMYEV)genome was amplified by 3’ rapid amplification of cDNA ends(RACE).Ten Chinese isolates were sequenced,and 7 of them were the same.Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world.Sequence analysis of the 878 nt stretch within 3’ terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%,deduced amino acid sequences of coat protein gene identity were 86.4% to 100%.Phylogenetic analysis showed that all isolates of SMYEV fell into four clades.To a certain extent,the clades were related with the geological distribution of SMYEV.Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates,but formed a small separate branch.Isolates SY03 and SY02,derived from Fragaria×ananassa cv.Changhong-2 and F.pentaphylla respectively,had a far relationship with other isolates and fell into one clade.They were likely to be the special isolates that existed only in China.
The 930 bp segment in 3 ’terminal region of Strawberry mild yellow edge virus (SMYEV) genome was amplified by 3’ rapid amplification of cDNA ends (RACE). Ten Chinese isolates were sequenced, and 7 of them were the same. Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world. Sequence analysis of the 878 nt stretch within 3 ’terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%, deduced amino acid sequences of coat protein gene identity were 86.4% to 100%. Phylogenetic analysis showed that all isolates of SMYEV fell into four clades. To certain extent, the clades were related with the geological distribution of SMYEV. Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates, but formed a small separate branch. Isolates SY03 and SY02, derived from Fragaria × ananassa cv. Changhong-2 and F.pentaphylla respectively, had a far relationship with oth er isolates and fell into one clade. They were likely to be the special isolates that existed only in China.