论文部分内容阅读
大豆疫霉根腐病是大豆破坏性病害之一。利用抗病品种是防治该病的唯一有效的方法。迄今,已经鉴定了15个抗大豆疫霉根腐病基因(Rps基因),而且大豆部分基因都获得了不同类型的分子标记。本研究根据以前抗病基因推导结果选择26个可能含有Rps1基因座位等位基因或(和)Rps4基因的大豆品种(系),利用分别与Rps1a和Rps4紧密连锁的SSR标记进行抗病基因的分子检测,通过比较含有已知抗病基因对照大豆品种(系)分子标记检测结果并综合以往基因推导结果推断检测品种(系)的Rps基因。在选择的26个品种(系)中,长农14号被证明含有Rps1a,周豆13和铁95068-5含有Rps1a与Rps4基因组合,品系50794、科8924-3和合豆1号含有Rps4;有11个品种(系)存在含有Rps1a的证据、品系50052被推断含有Rps1c与Rps3b基因组合,但不排除这些品种(系)在Rps1座位含有一个新等位基因的可能性。另外,有7个品种(系)的所含抗病基因不能确定,它们可能含有新的Rps基因。
Phytophthora sojae is one of the devastating diseases of soybean. The use of resistant varieties is the only effective way to control the disease. So far, 15 genes against root blight of Phytophthora sojae (Rps gene) have been identified, and different types of molecular markers have been obtained for some of the soybean genes. In this study, we selected 26 soybean cultivars (lines) which may contain the Rps1 allele or (and) Rps4 gene according to the deduced results of previous disease-resistance genes and used the SSR markers closely linked to Rps1a and Rps4 respectively to carry molecular resistance genes The Rps genes of the tested cultivars (lines) were inferred by comparing the results of molecular marker tests with the control soybean lines (lines) containing the known disease-resistance genes and synthesizing the deduced results of past genes. Among the 26 cultivars (lines) selected, Changnong 14 was proved to contain Rps1a, Zhou bean 13 and 95068-5 with Rps1a and Rps4 genes. Lines 50794, 8924-3 and Hetian No.1 contain Rps4; There were evidence of Rps1a in 11 cultivars. Line 50052 was inferred to contain a combination of Rps1c and Rps3b genes, but did not rule out the possibility that these cultivars would contain a new allele at the Rps1 locus. In addition, there are 7 varieties (lines) containing the disease-resistant genes can not be determined, they may contain a new Rps gene.