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目的通过16S rRNA基因序列分析快速鉴定艾伯特埃希菌。方法以30株疑似艾伯特埃希菌为材料,使用通用引物扩增其16S rRNA基因并进行测序。获得的序列与艾伯特埃希菌型菌株LMG 20976,基因组测序菌株KF1、NBRC107761、TW07627以及其他密切相关的细菌种(大肠埃希菌、赫曼埃希菌、费格森埃希菌、志贺菌和Shimwellia blattae)的16S rRNA基因序列进行比较,并使用N-J法构建进化树来分析其相关性。结果 30株疑似艾伯特埃希菌的16S rRNA基因序列与艾伯特埃希菌参考菌株的序列相似性最高,并与其聚类为一簇,为艾伯特埃希菌。结论 16S rRNA基因测序分析可用于艾伯特埃希菌的快速鉴定。
OBJECTIVE: To rapidly identify E.aei by 16S rRNA gene sequence analysis. Methods Thirty 30 strains of suspected Escherichia coli were used as primers, and their 16S rRNA genes were amplified by universal primers and sequenced. The sequences obtained were identical to the strains of Escherichia Alberti LMG 20976, the genomic sequencing strains KF1, NBRC107761, TW07627 and other closely related bacterial species (Escherichia coli, Hermansella, Feigei, Hela and Shimwellia blattae) 16S rRNA gene sequences were compared and NJ method was used to construct the phylogenetic tree to analyze the correlation. Results The sequence of 16S rRNA gene from 30 suspected strains of Escherichia coli was the most similar to that of the reference strain of Escherichia coli, and clustered into a cluster of Escherichia coli. Conclusion 16S rRNA gene sequencing analysis can be used for the rapid identification of Escherichia coli.