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作者应用地高辛配基标记HBV DNA探针进行原位杂交,检测16例(重症肝炎10例、HBsAg阳性3例、非肝脏疾病3例)肝组织标本内HBV DNA。结果表明:HBV DNA阳性物质在肝细胞内的分布有全胞浆型、包涵体型、全胞核型及核仁-核膜型4种方式。进一步说明地高辛配基标记探针原位杂交可以在一定程度上反映HBV DNA在细胞内的存在状态。作者将地高辛配基标记HBV DNA探针原位杂交与生物素标记探针进行比较,结果前者较后者灵敏度高、特异性强、实用性好,是理想的检测HBV DNA的新一代非同位素探针。
The authors used digoxigenin-labeled HBV DNA probe for in situ hybridization and detected HBV DNA in 16 cases (10 cases of severe hepatitis, 3 cases of HBsAg positive, 3 cases of non-liver disease). The results showed that there were four types of distribution of HBV DNA positive cells in the hepatocytes: whole cytoplasm, inclusion body, whole cell nucleus and nucleolus - nuclear membrane. To further explain the in situ hybridization of digoxigenin-labeled probe can reflect the existence of HBV DNA in the cells to some extent. The authors compared digoxigenin-labeled HBV DNA probe in situ hybridization with biotin-labeled probe, the results of the former than the latter high sensitivity, specificity, good practicability, is the ideal detection of a new generation of HBV DNA non- Isotopic probe.