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目的观察转化生长因子β1(TGF-β1)抗体对二氧化硅(SiO2)刺激的小鼠肺成纤维细胞(L929细胞)TGF-β1及Ⅰ型胶原表达的影响,探讨外源性TGF-β1抗体在抗肺纤维化中的作用。方法将L929细胞分为对照组、SiO2组和TGF-β1抗体组。对照组只加入RPMI-1640培养液3 ml,SiO2组、TGF-β1抗体组分别加入质量浓度为100.0mg/L的SiO23 ml,间隔2 h后,TGF-β1抗体组再加入质量浓度为1.0 mg/L的TGF-β1抗体1 ml,培养24 h后,采用酶联免疫吸附测定法检测L929细胞TGF-β1水平,免疫组化法检测TGF-β1及Ⅰ型胶原蛋白表达水平。结果 3组TGF-β1水平及蛋白表达水平分别比较,差异均有统计学意义(P<0.05);SiO2组TGF-β1水平和蛋白表达水平均高于对照组[(16.15±1.10)vs(9.15±0.44),(73.81±10.80)vs(18.42±3.48),P<0.05],TGF-β1抗体组TGF-β1水平和蛋白表达水平均低于SiO2组[(12.56±0.36)vs(16.15±1.10),(52.09±9.43)vs(73.81±10.80),P<0.05]。3组间Ⅰ型胶原表达水平比较,差异有统计学意义(P<0.05);SiO2组Ⅰ型胶原表达水平高于对照组[(42.36±8.34)vs(7.64±2.67),P<0.05],TGF-β1抗体组Ⅰ型胶原表达水平低于SiO2组[(29.81±7.45)vs(42.36±8.34),P<0.05]。结论 TGF-β1抗体可降低L929细胞中TGF-β1水平,并降低TGF-β1蛋白及Ⅰ型胶原蛋白的表达。
Objective To investigate the effect of transforming growth factor β1 (TGF-β1) on the expression of TGF-β1 and type Ⅰ collagen in mouse lung fibroblast (L929) cells stimulated with SiO2. Role in anti-pulmonary fibrosis. Methods L929 cells were divided into control group, SiO2 group and TGF-β1 antibody group. In the control group, only 3 ml of RPMI-1640 culture medium was added. The SiO 2 group and the TGF-β 1 antibody group were respectively added with SiO 2 3 ml with a mass concentration of 100.0 mg / L. After an interval of 2 h, the TGF-β1 antibody group was further added with 1.0 mg / L of TGF-β1 antibody 1 ml, cultured for 24 h, the level of TGF-β1 in L929 cells was detected by enzyme-linked immunosorbent assay, and the expression of TGF-β1 and type Ⅰ collagen was detected by immunohistochemistry. Results The levels of TGF-β1 and protein expression in the three groups were significantly different (P <0.05). The levels of TGF-β1 and protein in the SiO2 group were significantly higher than those in the control group [(16.15 ± 1.10) vs (9.15 The levels of TGF-β1 and TGF-β1 in the TGF-β1 group were significantly lower than those in the SiO2 group [(12.56 ± 0.36) vs (16.15 ± 1.10 ± 0.44), (73.81 ± 10.80 vs 18.42 ± 3.48, P <0.05) ), (52.09 ± 9.43) vs (73.81 ± 10.80), P <0.05]. (P <0.05). The expression of type I collagen in the SiO2 group was significantly higher than that in the control group (42.36 ± 8.34 vs 7.64 ± 2.67, P <0.05) The expression of type Ⅰ collagen in TGF-β1 antibody group was lower than that in SiO2 group [(29.81 ± 7.45) vs (42.36 ± 8.34), P <0.05]. Conclusion TGF-β1 antibody can reduce the level of TGF-β1 in L929 cells and decrease the expression of TGF-β1 and type Ⅰ collagen.