角质形成细胞具有广泛的生物学特征 ,对皮肤的结构和功能有着重要的影响。其体外培养技术的建立至今已有近 3 0年的历史。从液体浸没培养到空气 -液体交界面培养 ;从单层细胞培养到复层器官型培养及器官培养 ,形成了多种培养模型可供实验研究和临床应用。当今角质形成细胞的培养技术已广泛应用于皮肤生理学、病理学、药理学、药物毒理学、组织工程学和基因治疗等方面。介绍角质形成细胞体外培养的生长条件和环境、体外培养方法和应用三方面的最新进展。 Keratinocytes have a wide range of biological characteristics, the structure and function of the skin has a significant impact. Its in vitro culture technology has been established nearly 30 years of history. From liquid immersion culture to air-liquid interface culture, a variety of culture models have been developed for experimental studies and clinical applications, from single-cell cultures to multi-layered organotypic and organotypic cultures. Current keratinocyte culture techniques have been widely used in dermatology, pathology, pharmacology, drug toxicology, tissue engineering and gene therapy. The latest progress of keratinocytes in vitro culture conditions and environment, in vitro culture methods and application of three aspects.