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目的:以HLA高分辨测序分型技术为平台,调查广州脐血库HLA-Cw、DPB1、DQB1等位基因的多态性及HLA等位基因分布规律,建立相应的基因遗传数据分析资料,并为临床移植提供必要的HLA分型数据。方法:采用基于测序的PCR技术(sequencing based typeSBT),对广州脐血库128份脐血进行HLA-Cw、DPB1、DQB1等位基因测序分型。结果:HLA-Cw共检出20个等位基因型,其中HLA-Cw*0102基因频率最高,达22.70%;HLA-DQB1共检出16种等位基因型,其中HLA-DQB1*0301基因频率最高,达24.20%;HLA-DPB1共检出20种等位基因型,其中HLA-DPB1*0501基因频率最高,达41.0%。统计分析表明Cw、DPB1、DQB1位点的基因分布符合Hardy-weinburg平衡(P=0.081,0.156和0.079,均>0.05)。结论:统计数据显示HLA-Cw、DPB1、DQB1位点处于遗传平衡状态,具有较丰富多态性。广州脐血库HLA-Cw及HLA-DQB1等位基因分布与中国北方人群差异无统计学意义,而HLA-DPB1等位基因分布与中国北方人群差异有统计学意义。
OBJECTIVE: To investigate HLA polymorphisms and HLA alleles distribution of HLA-Cw, DPB1 and DQB1 genes in Guangzhou umbilical cord blood bank using HLA high resolution sequencing and typing technique as the platform to establish the corresponding genetic data analysis data Clinical transplantation provides the necessary HLA typing data. Methods: Sequencing-based PCR (sequencing based type SBT) was used to screen the HLA-Cw, DPB1 and DQB1 alleles of 128 cord blood in Guangzhou cord blood bank. Results: Twenty alleles were detected in HLA-Cw, of which HLA-Cw * 0102 was the highest (22.70%). HLA-DQB1 was detected in 16 alleles, of which HLA-DQB1 * 0301 Up to 24.20%. Twenty alleles were detected in HLA-DPB1, of which HLA-DPB1 * 0501 was the highest (41.0%). Statistical analysis showed that the distribution of Cw, DPB1 and DQB1 loci was in accordance with Hardy-weinburg equilibrium (P = 0.081,0.156 and 0.079, both> 0.05). Conclusion: The statistical data show that HLA-Cw, DPB1 and DQB1 loci are in a state of genetic equilibrium with rich polymorphism. The distribution of HLA-Cw and HLA-DQB1 alleles in Guangzhou umbilical cord blood bank was not significantly different from those in northern China, but the allele distribution of HLA-DPB1 was significantly different from that in northern China.