An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip. The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol/L for glucose and 4.5 mol/L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month. An amperometric multianalyte biosensor for the simultaneous determination of glucose and galactose was de-veloped based on chamber-type electrodes, which were fabricated by micromachining technology. The dual cham-ber-type enzyme electrode with glucose and galactose sensor elements was integrated onto one microchip . The ex-perimental parameters of this biosensor were optimized. The biosensor exhibited a linearity of up to 4.0 mol / L for glucose and 4.5 mol / L for galactose, and the response time was about 30 s for glucose and 40 s for galactose. No cross-talking behavior was investigated in the course of simultaneous measurement of the two analytes. Interference from electroactive species, such as ascorbic acid and uric acid, was minimized due to the permselectivity of Nafion film. In addition, the biosensor displayed a storage stability of longer than one month.