以无花果为组织培养试验材料,采用改变培养条件和使用不同培养基的方法,研究培养条件对外植体褐化的影响。结果表明:无花果外植体褐化在WPM培养基上比MS培养基上较轻,培养基中琼脂为9g的无花果外植体褐化较轻,经过暗处理的无花果外植体褐化程度较轻,以及低温条件能有效缓解外植体褐化。 In order to study the effect of culture conditions on the browning of explants, the figs were used as the tissue culture materials, and the culture conditions were changed and different media were used. The results showed that the browning of fig explants was lighter than that of MS medium on WPM medium, and the explants of 9g agar had less browning on the medium. Light, as well as low temperature conditions can effectively alleviate the browning of explants.