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由健康人外周血分离淋巴细胞及溶血处理后的全白细胞,以外源性表皮生长因子和甲硫氨酸脑啡肽培育后取细胞悬液制成滴片和滴膜,用生物素标记的c-fos cDNA探针进行原位杂交和斑点印迹杂交.结果显示表皮生长因子和甲硫氨酸脑啡肽两组c-fos原癌基因的表达均较对照组增强(P<0.05).
From healthy human peripheral blood lymphocytes and hemolysis after treatment of leukocytes to exogenous epidermal growth factor and methionine enkephalin after the cell suspension was taken to make slides and drip membrane biotinylated c -fos cDNA probe was used for in situ hybridization and dot-blot hybridization.The results showed that the expression of c-fos proto-oncogene in both epidermal growth factor and methionine enkephalin groups were enhanced compared with the control group (P <0.05).