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目的了解石家庄市2010年-2012年手足口病(HFMD)病原情况及柯萨奇病毒A16型(CA16)病毒的基因特征,为HFMD防控提供参考。方法对临床诊断为HFMD的病例标本,采用Real-time RT-PCR进行肠道病毒核酸检测;部分CA16型阳性标本进行VP1区全长测序,采用MEGA 5.0进行序列比对和进化树构建。结果在3173份标本中,2556份标本检测为通用肠道病毒核酸阳性,其中CA16阳性标本851份。2010年-2012年石家庄流行的CA16病毒株为B1基因型,2010年为B1a亚型,2011年和2012年出现了B1a和B1b两种基因亚型共循环现象,且存在多个传播链。结论石家庄市来源于HFMD的CA16流行病毒株属于B1基因型,存在B1a和B1b两种基因亚型共循环现象。
Objective To understand the pathogen status of HFMD and the genetic characteristics of Coxsackievirus A16 type (CA16) virus from 2010 to 2012 in Shijiazhuang and to provide a reference for the prevention and control of HFMD. Methods Real-time RT-PCR was used to detect the cases of HFMD. Some CA16 positive specimens were sequenced for the full-length of VP1 region. MEGA 5.0 was used for sequence alignment and phylogenetic tree construction. Results Among 3173 specimens, 2556 specimens were tested positive for universal enterovirus, including 851 CA16 positive specimens. From 2010 to 2012, the epidemic CA16 strain in Shijiazhuang was B1 genotype, B1a subtype in 2010, and B1a and B1b common circulatory phenotype in 2011 and 2012, and there were multiple transmission chains. Conclusions The CA16 epidemic strain originating from HFMD in Shijiazhuang belongs to the B1 genotype, and there exists a phenomenon of co-circulation of two gene subtypes, B1a and B1b.