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目的探讨蛋白酶激活受体2(PAR-2)在过敏性休克中的作用,为过敏性休克的治疗提供新的思路。方法昆明种雄性小鼠随机分成致敏组和对照组。致敏组小鼠于第1天腹腔注射致敏原,1周后加强注射1次,饲养3周后尾静脉注射致敏原诱发过敏性休克。对照组以同等量的PBS缓冲液代替。酶联免疫吸附法(ELISA)检测致敏组和对照组小鼠血清类胰蛋白酶及类糜蛋白酶含量;RT-PCR法检测血液中PAR-2相对表达量。结果成功建立过敏性休克小鼠模型,RT-PCR结果显示致敏后小鼠PAR-2 m RNA相对表达量为3.372±0.28,对照组为0.759±0.062,2组比较有显著性差异(P<0.01);ELISA结果显示致敏后小鼠血清类胰蛋白酶含量为72.378μg/L±8.018μg/L,正常对照组为20.265μg/L±1.953μg/L,2组比较有显著性差异(P<0.01),致敏后小鼠血清类糜蛋白酶含量(16.186μg/L±0.959μg/L)低于对照组(34.905μg/L±3.972μg/L),组间比较有显著性差异(P<0.01)。结论 PAR-2可能参与了过敏性休克的发病过程。
Objective To investigate the role of PAR-2 in anaphylactic shock and provide a new idea for the treatment of anaphylactic shock. Methods Kunming male mice were randomly divided into sensitized group and control group. The sensitized mice were injected intraperitoneally with allergens on day 1, and once a week later, the mice were injected with allergens to induce anaphylactic shock. The control group was replaced with the same amount of PBS buffer. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum tryptase and chymase in the sensitized group and control group. The relative expression of PAR-2 in blood was detected by RT-PCR. Results The model of anaphylactic shock was established successfully. RT-PCR results showed that the relative expression of PAR-2 mRNA in sensitized mice was 3.372 ± 0.28, and the control group was 0.759 ± 0.062. There was significant difference between the two groups (P < 0.01). The result of ELISA showed that the serum tryptase content of the mice was 72.378μg / L ± 8.018μg / L after sensitization and 20.265μg / L ± 1.953μg / L in the normal control group (P (P <0.01). The serum chymase content of mice after immunization was lower than that of the control group (16.186μg / L ± 0.959μg / L, 34.905μg / L ± 3.972μg / L, P <0.01). Conclusion PAR-2 may be involved in the pathogenesis of anaphylactic shock.