论文部分内容阅读
为了明确来自湖北武穴、孝感、随州3个不同地区的转cry1Ab/cry1Ac、cry2A、cry1C基因明恢63(分别命名为TT51、T2A-1和T1C-19)对赤拟谷盗Tribolium castaneumHerbst生长发育的影响,在室内以不同的转Bt基因稻谷继代饲养赤拟谷盗6代,结果表明:各处理赤拟谷盗的卵期3～4d,卵孵化率85%～100%,幼虫期22～27d,化蛹率85%～97%,蛹期6～7d,百蛹重0.25～0.31g,性比0.8～1.4,羽化率82%～97%,产卵前期5～7d。不同抗虫转基因水稻对赤拟谷盗各发育历期及生命表参数没有显著的影响,同一转基因事件,没有因为种植地区的不同造成对赤拟谷盗生长发育的差异。3种Bt蛋白在继代饲养的赤拟谷盗幼虫体内检测均呈阳性反应,但积累量很小,继代饲养后,没有发现在赤拟谷盗体内明显的累积。
In order to clarify the growth and development of Tribolium castaneum Herbst from Cry1Ab / cry1Ac, cry2A and cry1C genes Minghui63 (named as TT51, T2A-1 and T1C-19 respectively) in 3 different areas of Hubei Province, The results showed that the eggs of each treatment were 3 ~ 4 days old, the hatching rate of eggs was 85% ~ 100%, and the larval period of 22 ~ 27d, the pupation rate is 85% ~ 97%, the pupal stage is 6 ~ 7d, the pupal weight is 0.25 ~ 0.31g, the sexual ratio is 0.8 ~ 1.4, the emergence rate is 82% ~ 97% Different insect-resistant transgenic rice had no significant effect on the developmental stages and life table parameters of Brassica parachinensis. The same transgenic event did not result in the difference in the growth and development of Brassica parachinensis due to the different planting areas. All the three Bt proteins showed positive reaction in the subculture of C. rubescens larvae, but the accumulated amount was very small. No significant accumulation in the body of Cochrane was observed after subculture.