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目的：建立测定生长激素（ＧＨ）在体生物活性的方法．方法：以去垂体大鼠体重增长（ＢＷＧ）和胫骨骺软骨板宽度（ＴＥＷ）为指标，观察动物性别、给药途径、次数和周期不同对效应的影响；同时进行４ｄＢＷＧ，６ｄＢＷＧ和６ｄＴＥＷ法，测定ＧＨ的效价（平行线３×３设计）．结果：♀和♂ｓｃ和ｉｍ给药以及每日给药１次和２次的ＢＷＧ和ＴＥＷ差异无显著意义．给药６ｄ比给药４ｄ引起较大的ＢＷＧ和ＴＥＷ（Ｐ＜００５）．４ｄＢＷＧ法和６ｄＢＷＧ法在００２０－０５００ＩＵ·ｄ－１有较好的λ值（００６６０和０１７４７）和ｒ值（０９０００和０９２３７）；４ｄＢＷＧ，６ｄＢＷＧ和６ｄＴＥＷ法测得ｒｈＧＨ的效价为４６１３２，３９８２９和４８０２３ＩＵ／ａｍｐ．６ｄＢＷＧ法有较小的λ值和较低的ＡＲＦＬ值．结论：可在同一组去垂体大鼠体内同时用４ｄＢＷＧ，６ｄＢＷＧ和６ｄＴＥＷ法测ＧＨ活性，以６ｄＢＷＧ法较好．
Objective: To establish a method for determining the in vivo biological activity of growth hormone (GH). Methods: The effects of different sex, route of administration, number of times and cycles on the effect were observed by weight gain (BWG) and epiphyseal cartilage plate width (TEW) of the pituitary gland. At the same time, 4dBWG and 6dBWG And 6 dTEW method, determination of GH titer (parallel lines 3 × 3 design). RESULTS: There was no significant difference in BWG and TEW between ♀ and ♂ sc and im administration, and once and twice daily dosing. Administration of 6d resulted in greater BWG and TEW than 4d administration (P <005). 4-dBWG method and 6-dBWG method have better λ values (00660 and 01747) and r values (09000 and 09237) at 020-0500 IU · d-1; 4 The titers of rhGH measured by dBWG, 6dBWG and 6dTEW methods were 4.132, 3.9829 and 4.8023IU / amp. 6 dBWG method has a smaller λ value and lower ARFL value. Conclusion: In the same group of pituitary rats in vivo at the same time with 4 dBWG, 6 dBWG and 6 dTEW method to measure GH activity, 6 dBWG method is better.