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研究了在紫外光作用下,牛血清白蛋白(BSA)在偶氮苯自组装膜上光控可逆的吸附行为.首先合成羧基偶氮苯衍生物,并在金膜表面制备偶氮苯自组装膜,采用紫外吸收光谱(UV)、原子力显微镜(AFM)观察偶氮苯衍生物的光学顺反异构现象以及偶氮苯自组装膜表面形貌的变化.同时利用等离子体表面谐振仪(SPR)考察偶氮苯光学异构对牛血清白蛋白(BSA)在自组装膜表面上的吸附行为的影响.结果表明,BSA在偶氮苯自组装膜表面的吸附作用主要来自于BSA分子与自组装膜之间的静电作用及亲疏水作用.在紫外光作用下,偶氮苯自组装膜可以实现光控可逆的牛血清白蛋白分子吸附行为.
The photocatalytic reversible adsorption behavior of bovine serum albumin (BSA) on the azobenzene self-assembled monolayers was studied under the action of ultraviolet light.At first, carboxyl azobenzene derivatives were synthesized and the azobenzene self-assembly The optical cis-trans isomerization of azobenzene derivatives and the surface topography of Azobenzene self-assembled films were observed by UV-Vis absorption spectroscopy (UV-Vis) and atomic force microscopy (AFM) ) Was used to investigate the effect of optical isomerisation of azobenzene on the adsorption behavior of bovine serum albumin (BSA) on the surface of self-assembled monolayers.The results showed that the adsorption of BSA on the surface of azobenzene self- The electrostatic interaction between the membrane and the hydrophilicity and hydrophobicity of the membrane.Ultraviolet-induced azobenzene self-assembled monolayers can realize the photo-controllable reversible adsorption behavior of bovine serum albumin.