论文部分内容阅读
对巨细胞病毒(CMV)感染的血清学诊断及胎儿出生前后感染该病毒的检查,可测定抗病毒非结构蛋白抗体,即抗早期抗原的抗体。测定这种抗体,需要含有CMV早期抗原的细胞制剂,该抗原由感染含阿糖胞苷(AraC)的人成纤维细胞而得到。AraC能抑制DNA的合成,从而阻止了病毒的复制及病毒结构蛋白(即晚期抗原)的形成。已知用人CMV感染兔肺成纤维细胞,能诱导早期抗原,而无病毒复制。作者用感染兔成纤维细胞和感染后经AraC处理的人成纤维细胞,测定急性CMV感染病人与健康人血清中抗CMV早期抗原
Serological diagnosis of cytomegalovirus (CMV) infection and fetal infection before and after infection with the virus can detect anti-virus non-structural protein antibodies, anti-early antigen antibodies. To determine such an antibody, a cell preparation containing CMV early antigen is needed, which antigen is obtained from human fibroblasts infected with cytarabine (AraC). AraC can inhibit the synthesis of DNA, thus preventing the replication of the virus and the formation of viral structural proteins (ie, late antigens). It is known that infection of rabbit lung fibroblasts with human CMV induces early antigens without viral replication. The authors infected and infected with rabbit fibroblasts after AraC-treated human fibroblasts in patients with acute CMV infection and healthy serum anti-CMV early antigen