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目的:建立环磷酰胺(CTX)治愈荷膀胱癌T739小鼠的动物模型,观察外周血象特别是血小板计数在化疗治愈肿瘤中的动态变化及其意义。方法:给正常T739小鼠皮下接种小鼠可移植性膀胱移行细胞癌组织,建立荷膀胱癌的小鼠模型。接种后第7天,荷瘤小鼠腹腔内分别注入15、40、100mg/kg的CTX,等量生理盐水对照,确定CTX治愈肿瘤的量效关系。然后再取12只荷瘤小鼠,随机分成2组,15mg/kgCTX单次腹腔内注射,等量生理盐水对照,分别在用药后6h、用药后第2、4、9、14天内眦静脉取血,进行血常规检测,分析荷瘤小鼠外周血象的动态变化。结果:15～100mg/kgCTX治疗后2周内,荷瘤小鼠肿瘤生长受抑制或肿瘤结节缩小的速率与所用CTX的剂量呈正相关;CTX治疗后2月,荷瘤小鼠的存活率与所用CTX的剂量呈负相关。15mg/kgCTX单次腹腔内注射在治愈多数荷瘤小鼠的同时对外周血象无明显抑制作用。用药后6h治疗组荷瘤鼠外周血血小板计数为(1483.4±184.4)×109/L,明显高于对照组小鼠(1086.6±81.0)×109/L,两组数据比较具有统计学意义(P<0.01);用药后第2、4、9、14天治疗组荷瘤鼠外周血血小板计数与对照组小鼠比较差异无统计学意义(P>0.05)。结论:15mg/kgCTX可治愈多数荷膀胱癌T739小鼠。单剂量CTX治疗后6h荷瘤小鼠外周血血小板计数的一过性增高可能与其抗肿瘤作用有关。
OBJECTIVE: To establish an animal model of bladder cancer T739 induced by cyclophosphamide (CTX) and observe the dynamic changes of peripheral blood, especially platelet count, in chemotherapy-cured tumors. Methods: T739 mice were inoculated subcutaneously with transplanted bladder transitional cell carcinoma of mice to establish a mouse model of bladder cancer. On the 7th day after inoculation, CTX of 15, 40 and 100 mg / kg were injected intraperitoneally into the tumor-bearing mice, and the same amount of saline control was injected into the tumor-bearing mice to determine the dose-response relationship of CTX in curing the tumor. Then, 12 tumor-bearing mice were randomly divided into 2 groups, 15mg / kg CTX single intraperitoneal injection, the same amount of saline control, respectively, 6h after treatment, the first 2,4,9,14 days after treatment, Blood, blood tests, analysis of tumor-bearing mice peripheral blood changes. Results: The rate of tumor growth inhibition or tumor nodules shrinking was positively correlated with the dose of CTX used within 2 weeks after 15 ~ 100mg / kg CTX treatment. After 2 months of CTX treatment, the survival rate of tumor-bearing mice The dose of CTX used was negatively correlated. A single intraperitoneal injection of 15mg / kgCTX in the treatment of most tumor-bearing mice without significant inhibition of peripheral blood. The platelet count of peripheral blood of the treated group was (1483.4 ± 184.4) × 109 / L at 6h after treatment, which was significantly higher than that of the control group (1086.6 ± 81.0) × 109 / L, the data of the two groups were statistically significant (P <0.01). There was no significant difference in platelet counts between the treated group and the control group on the 2nd, 4th, 9th, 14th day after treatment (P> 0.05). Conclusion: Most bladder cancer T739 mice can be cured by 15mg / kg CTX. A transient increase of platelet count in peripheral blood of 6-h tumor-bearing mice after single-dose CTX treatment may be related to its antitumor effect.