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研究了用2-(2-喹啉偶氮)-5-二乙氨基酚(QADEAP)为柱前衍生试剂,甲醇(内含0.2%醋酸)和pH4.0醋酸-醋酸钠缓冲溶液梯度洗脱为流动相,WatersNova-Pak-C18液相色谱柱为固定相,二极管矩阵检测器检测,高效液相色谱法分离测定食品中铁、钴、镍、铜、锌和锰的的方法。根据信噪比(S/N=3)各金属离子的检测限分别为:铁3.2μg/L,钴4.1μg/L,镍2.6μg/L,铜4.4μg/L,锌5.0μg/L,锰8.0μg/L,方法相对标准偏差在1.6%～3.5%之间,标准回收率在93%～107%,方法用于食品样品中痕量铁、钴、镍、铜、锌、锰的测定,结果令人满意。
A gradient elution of 2- (2-quinolyl azo) -5-diethylaminophenol (QADEAP) as a pre-column derivatization reagent in methanol (containing 0.2% acetic acid) and pH 4.0 acetate- As mobile phase, WatersNova-Pak-C18 column was used as the stationary phase, diode array detector was used for the determination of iron, cobalt, nickel, copper, zinc and manganese in food by high performance liquid chromatography. The detection limits of each metal ion were 3.2μg / L for Fe, 4.1μg / L for cobalt, 2.6μg / L for nickel, 4.4μg / L for copper, 5.0μg / L for zinc, The method was applied to the determination of trace iron, cobalt, nickel, copper, zinc and manganese in food samples with the standard deviation of 1.6% ~ 3.5% and the standard recovery of 93% -107%. , The result is satisfactory.