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目的探讨延龄草苷(trillin)对小胶质细胞(BV-2)的炎性激活的抑制作用。方法不同浓度的延龄草苷孵育0.5h,然后用脂多糖进行刺激,培养24 h后收集上清,Greiss法检测NO浓度;CCK-8检测延龄草苷对小胶质细胞活力的影响;Elisa检测肿瘤坏死因子(TNF-α)的浓度;实时定量PCR(RT-PCR)检测TNF-α、IL-1β、IL-6、iNOS、COX-1、COX-2基因的表达。结果在不影响细胞活力的前提下,延龄草苷能明显抑制NO的释放,同时能抑制激活诱导细胞死亡,能明显抑制TNF-α的产生,能明显抑制TNF-α、IL-1β、IL-6、iNOS、COX-2 mRNA的表达,但是不能抑制COX-1 mRNA的表达。结论延龄草苷可以抑制LPS诱导的小胶质细胞的炎性激活,抑制炎性基因的表达。
Objective To investigate the inhibitory effect of trillin on inflammatory activation of microglia (BV-2). Methods Trichosanthin at different concentrations was incubated for 0.5 h and then stimulated with lipopolysaccharide. The supernatants were collected after 24 h of culture. The concentrations of NO were measured by Greiss method. The effect of trillin on the viability of microglial cells was determined by CCK-8. The expression of TNF-α, IL-1β, IL-6, iNOS, COX-1 and COX-2 were detected by real-time quantitative PCR (RT- Results Without affecting cell viability, trillin glycosides could significantly inhibit the release of NO and at the same time inhibit the activation-induced cell death, significantly inhibit the production of TNF-α, and significantly inhibit the expression of TNF-α, IL-1β, IL -6, iNOS, COX-2 mRNA expression, but can not inhibit the expression of COX-1 mRNA. Conclusion Trillium glycosides can inhibit LPS-induced inflammatory activation of microglia and inhibit the expression of inflammatory genes.