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目的 研究舌癌组织中血管内皮生长因子C(vascular endothelial growth factor C VEGF-C)及其受体VEGFR-3(flt-4)的表达情况,分析其在肿瘤淋巴转移中的作用。方法 采用RT-PCR法分析22例新鲜舌癌标本VEGF-C/flt-4mRNA的表达情况,免疫组化SP法分析55例石蜡标本中VEGF-C/flt-4蛋白表达情况,统计分析其与临床病理特点之间的关系。结果 VEGF-CmRNA表达阳性率为72.7％(16/22),VEGF-C和flt-4mRNA的表达高度一致(p<0.01)。VEGF-C蛋白阳性率为52.7％(29/55),明显高于正常组织和良性病变。与肿瘤病理分级、肿瘤颈淋巴结转移显著相关;flt-4的阳性率为41％(23/55),和VEGF-C阳性表达高度一致;VEGF-C阳性组5年生存率显著低于VEGF-C阴性组。结论 VEGF-C/flt4在舌癌表达高于正常组织和良性病变;与肿瘤的预后密切相关。在肿瘤淋巴转移中可能丘重要作用。
Objective To study the expression of vascular endothelial growth factor C (VEGF-C) and its receptor VEGFR-3 (flt-4) in tongue cancer and its role in tumor lymphatic metastasis. Methods RT-PCR was used to analyze the expression of VEGF-C / flt-4 mRNA in 22 cases of fresh tongue cancer specimens. The expression of VEGF-C / flt-4 protein in 55 cases of paraffin specimens was analyzed by immunohistochemical SP method. The relationship between clinicopathological features. Results The positive rate of VEGF-C mRNA expression was 72.7% (16/22), and the expression of VEGF-C and flt-4 mRNA was highly consistent (p <0.01). The positive rate of VEGF-C protein was 52.7% (29/55), which was significantly higher than that of normal tissues and benign lesions. The positive rate of flt-4 was 41% (23/55), which was highly consistent with the positive expression of VEGF-C. The 5-year survival rate of VEGF-C positive group was significantly lower than that of VEGF- C negative group. Conclusion The expression of VEGF-C / flt4 in tongue cancer is higher than that in normal tissues and benign lesions, which is closely related to the prognosis of the tumor. May play an important role in tumor lymphatic metastasis.