采用紫外法测定发酵液中的L-赤藓酮糖。以谢里瓦诺夫(Seliwanoff)试剂为显色剂,考察了显色剂用量、反应温度、反应时间和显色稳定时间等因素,确定了优化后的试验条件。紫外法的线性范围为0~1.2 g/L,样品回收率为101.5%~101.9%,测定结果不受发酵液中底物及菌体自溶物影响,具有快速准确的优点。 Determination of L-erythrulose in fermentation broth by UV method. Taking Seliwanoff reagent as developer, the factors such as the amount of developer, reaction temperature, reaction time and color stability time were investigated, and the optimized experimental conditions were determined. UV linear range of 0 ~ 1.2 g / L, the sample recovery was 101.5% ~ 101.9%, the measured results from the fermentation broth and cells from the dissolved matter, with fast and accurate advantages.