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采用浸液法和药膜法测定了黄皮种子提取物对朱砂叶螨的生物活性。结果表明,黄皮种子甲醇粗提物对朱砂叶螨具有良好的触杀活性,其质量浓度为10 mg/mL时,对成螨和卵的校正死亡率分别为95.77%和98.32%,其LC50分别为0.876 3、1.2737 mg/mL;同时,对朱砂叶螨表现出良好的驱避和产卵抑制活性,在质量浓度为20 mg/mL时,对朱砂叶螨24 h的产卵抑制率和驱避率分别达到95.96%和88.79%。试验还表明,黄皮种子中的活性成分主要存在于石油醚中,其甲醇粗提物的石油醚萃取液质量浓度为2 mg/mL时,对朱砂叶螨成螨和卵的校正死亡率分别为100%和93.16%。
The biological activity of the extract of Huangpi on Tetranychus cinnabarinus was determined by immersion method and membrane method. The results showed that the crude methanol extracts from yellow seed had good contact activity against Tetranychus cinnabarinus, and the corrected mortality rates of adult mites and eggs at the concentration of 10 mg / mL were 95.77% and 98.32% 0.876 3,1.2737 mg / mL. At the same time, Spodoptera frugiperda showed good repellent and oviposition inhibiting activity. At the concentration of 20 mg / mL, The avoidance rates reached 95.96% and 88.79% respectively. The test also showed that the active ingredient in the yellow seed mainly exists in petroleum ether, the corrected mortality of mites and eggs of the Tetranychus cinnabarinus at the mass concentration of petroleum ether extract of methanol crude extract of 2 mg / mL is 100 % And 93.16%.