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目的:建立硫糖铝中铝离子(Al~(3+))在大鼠胃组织残留量的测定方法,考察硫糖铝对胃溃疡粘膜的靶向粘附作用。方法:采用干法灰化消解胃组织样品,电感耦合等离子体-原子发射光谱法(ICP-AES)测定给药后不同时间点大鼠胃组织中Al~(3+)的残留量。ICP-AES检测波长为396.153 nm,检测器为电感耦合阵列检测器,载气为氩气。结果:干法灰化大鼠胃溃疡组织,样品消解完全,可满足ICP-AES的检测要求;在选定的最佳条件下用ICP-AES检测Al~(3+)的检出限为8 ng·m L~(-1),样品间Al~(3+)残留量相对偏差小于13.4%,回收率在96.0%~99.5%之间;给药0.5~4 h时间段内,溃疡胃组织中的Al~(3+)残留率为正常胃组织的2倍以上;给药6 h后,正常胃组织中Al~(3+)残留率显著下降,仅为0.84%,而溃疡胃组织在给药16 h后仍高达29%。结论:ICP-AES测定大鼠胃组织中Al~(3+)的残留量准确可行;硫糖铝对胃溃疡粘膜的粘附性显著高于正常胃粘膜,具有良好的靶向性。
OBJECTIVE: To establish a method for the determination of aluminum ion (Al 3+) in sucralfate in rat gastric tissue, and to study the targeted adhesion of sucralfate to mucosa of gastric ulcer. Methods: Gastric tissue samples were digested with dry ashing method, and residual levels of Al 3+ in gastric tissues were determined by inductively coupled plasma-atomic emission spectrometry (ICP-AES) at different time points. ICP-AES detection wavelength of 396.153 nm, the detector is an inductively coupled array detector, the carrier gas is argon. Results: The gastric ulcer tissues of rat were ashed by dry method. The samples were completely digested and satisfied the ICP-AES detection requirements. The detection limit of Al 3+ by ICP-AES under the optimal conditions was 8 ng · m L -1, the relative deviation of Al 3+ residues was less than 13.4% and the recoveries were between 96.0% -99.5%. In the period of 0.5-4 h, the gastric ulcer tissues The residual rate of Al 3+ in normal gastric tissue was more than 2 times higher than that in normal gastric tissue at 6 hours. The residual rate of Al 3+ in normal gastric tissue decreased significantly at 0.84% Still up to 29% after 16 h. CONCLUSION: The determination of Al 3+ in gastric tissue by ICP-AES is accurate and feasible. The sucralfate adhesion to gastric ulcer mucosa is significantly higher than that of normal gastric mucosa, and has good targeting.