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采用间接免疫荧光标记法对玉米根细胞中的类整合素蛋白和细胞骨架主要组分之一的α-微管蛋白进行了荧光定位。结果表明:类整合素蛋白主要分布在质膜上。与对照相比,用与类整合素蛋白特异结合的5肽GRGDS处理后,质膜上类整合素的分布更为均匀,微管的排列密度降低,而用不与类整合素蛋白特异结合的GRGDS类似物SDGRG处理则对类整合素蛋白分布和微管蛋白的排列均无明显影响。微管蛋白解聚剂或稳定剂处理改变类整合素在质膜上的分布。这些结果表明类整合素蛋白与微管蛋白间有复杂的相互作用。
Indirect immunofluorescence labeling was used to detect the α-tubulin localization of integrins and one of the major components of cytoskeleton in maize root cells. The results showed that integrins were mainly distributed on the plasma membrane. Compared with the control, GRGDS, a 5-peptide specific binding to integrins, showed a more uniform distribution of integrins on the plasma membrane with a reduced array density of microtubules, whereas no specific binding to integrins GRGRDS analogue SDGRG treatment had no significant effect on the integrin protein distribution and tubulin arrangement. Tubulin depolymerization or stabilizing agents alter the distribution of integrins on the plasma membrane. These results indicate that integrins and tubulin have complex interactions.