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目的:扩增2009年镇江市肠道病毒71型(EV71)vp1基因的核苷酸序列,同相关毒株序列进行同源性比对和进化分析,以了解2009年镇江市肠道病毒71型的基因组序列特征及可能的传播来源。方法:采集临床手足口病(HFMD)患者咽拭子标本,抽提病毒RNA,通过巢式PCR扩增肠道病毒71型vp1基因的核苷酸序列。利用Clustal X和MEGA5.0分析软件,进行同源性分析和构建系统发生树。结果:序列进化分析表明,测序获得的11株病毒基因与C基因型代表株比较接近,尤其与C4基因亚型最为相近,并且可进一步划分为C4a进化枝。说明本文的11株病毒皆属于EV71病毒C基因型、C4a亚型。结论:2009年镇江市的EV71病毒株可能与阜阳分离的EV71病毒有相同的起源,均属于C4a亚型,并且C4a亚型的EV71病毒在中国大陆有较广泛的分布和传播。
OBJECTIVE: To amplify the nucleotide sequence of vp1 gene of enterovirus 71 (EV71) in Zhenjiang City in 2009 and to compare homology and evolution with the related strains so as to understand the sequence of EV71 Genomic sequence characteristics and possible sources of transmission. Methods: Throat swabs were collected from patients with HFMD. The viral RNA was extracted and the nucleotide sequence of vp1 gene of enterovirus 71 was amplified by nested PCR. Homology analysis and phylogenetic tree construction were performed using Clustal X and MEGA 5.0 software. Results: Sequence analysis showed that the 11 strains of viruses obtained by sequencing were close to the C genotype strains, especially the C4 subtype, and could be further divided into C4a clades. This shows that the 11 strains of viruses belong to EV71 virus C genotype, C4a subtype. CONCLUSION: The EV71 strain in Zhenjiang may have the same origin as the EV71 virus isolated in Fuyang in 2009, belonging to the C4a subtype, and the EV71 virus of the C4a subtype is widely distributed and spread in mainland China.